Frozen nqaij slicer sampling thiab kho tej yam kev paub

1. Txoj kev kho cov ntaub so ntswg me me: Qhov no yog ib txoj kev siv ntau. Cov ntaub so ntswg me me tshem tawm ntawm lub cev tsiaj los ntawm cov nqaij khov khov yuav tsum tau muab tso rau tam sim ntawd rau hauv cov kua dej fixative rau fixation. Feem ntau, qhov piv ntawm cov qauv mus rau fixative yog 1: 4 rau 20;

2. Steam fixation method: Rau cov qauv me thiab tuab, osmic acid los yog formaldehyde chav fixation txoj kev yuav siv tau. Xws li cov ntshav smear, nws yuav tsum tau kho nrog osmic acid los yog formaldehyde vapor ua ntej cov ntshav smear qhuav;

3. Thaum slicing nrog ib tug khov nqaij slicer, peb feem ntau siv fixatives yog 10% formaldehyde fixative thiab 95% ethanol fixative;

4. Kev txhaj tshuaj, perfusion fixation: qee cov ntaub so ntswg loj dhau los yog cov tshuaj fixative nyuaj rau nkag mus rau sab hauv, lossis tag nrho cov khoom nruab nrog cev lossis tag nrho cov tsiaj lub cev yuav tsum tau kho;

5. Siv kev txhaj tshuaj fixation lossis perfusion fixation, lub fixative yog txhaj rau hauv cov hlab ntsha, thiab cov hlab ntsha ceg mus rau tag nrho cov ntaub so ntswg thiab tag nrho lub cev, thiaj li yuav tau txais fixation txaus.