- 02
- Mar
Kev paub txog cov khoom siv thiab kho cov nqaij khov khov
Relevant knowledge of material and fixation of khov nqaij slicer
1. Txoj kev kho cov ntaub so ntswg me me: Qhov no yog ib txoj kev siv ntau. Cov ntaub so ntswg me me tshem tawm ntawm tus tsiaj los ntawm khov nqaij slicer yuav tsum tau muab tso rau tam sim ntawd rau hauv cov kua fixative rau fixation. Feem ntau, qhov piv ntawm cov qauv mus rau fixative yog 1: 4 ~ 20;
2. Steam fixation method: For relatively small and thick specimens, osmium acid or formaldehyde vapor fixation method can be used. For blood smears, osmium acid or formaldehyde vapor should be used to fix the blood before drying;
3. Thaum siv cov nqaij khov khov rau slicing, peb cov khoom siv feem ntau yog 10% formaldehyde fixative thiab 95% ethanol fixative;
4. Injection and perfusion fixation method: some tissue blocks are too large or the fixative is difficult to penetrate into the interior, or the entire organ or the entire animal body needs to be fixed;
5. Adopt injection fixation or perfusion fixation method to inject the fixative into the blood vessel and reach the whole tissue and the whole body through the branch of the blood vessel, so as to obtain sufficient fixation.
Txhawm rau ua kom tiav, kev xaiv thiab kho cov nqaij khov khov yuav tsum ua raws li qee yam kev cai. Cov khoom sib txawv muaj qhov sib txawv. Cov kev kho sib txawv xav tau cov khoom sib txawv thiab cov txiaj ntsig zoo. Ib yam li ntawd, raws li cov khoom siv thiab cov tuab ntawm cov nqaij khov yuav raug txiav, cov khoom siv tau tuaj yeem raug coj mus thiab kho.