- 02
- 3月
冻肉切片机材质及固定的相关知识
Relevant knowledge of material and fixation of 冷冻肉切片机
1、小组织固定法:这是一种常用的方法。 冷冻肉切片机从动物身上取出的小组织必须立即放入液体固定剂中进行固定。 通常标本与固定液的比例为1:4~20;
2. Steam fixation method: For relatively small and thick specimens, osmium acid or formaldehyde vapor fixation method can be used. For blood smears, osmium acid or formaldehyde vapor should be used to fix the blood before drying;
3、使用冷冻肉切片机切片时,我们常用的固定剂有10%甲醛固定剂和95%乙醇固定剂;
4. Injection and perfusion fixation method: some tissue blocks are too large or the fixative is difficult to penetrate into the interior, or the entire organ or the entire animal body needs to be fixed;
5. Adopt injection fixation or perfusion fixation method to inject the fixative into the blood vessel and reach the whole tissue and the whole body through the branch of the blood vessel, so as to obtain sufficient fixation.
综上所述,冷冻肉切片机的选择和固定需要遵循一定的要求。 不同的部分有不同的要求。 不同的固定方式需要不同的材料和天然优势。 同理,可根据待切的冷冻肉的种类和厚度,取料固定。