- 02
- Mar
Ihe ọmụma dị mkpa nke ihe onwunwe na nhazi nke slicer anụ oyi kpọnwụrụ
Relevant knowledge of material and fixation of Mpekere anụ oyi kpọnwụrụ
1. Usoro nhazi obere anụ ahụ: Nke a bụ usoro a na-ejikarị eme ihe. A ghaghị itinye obere anụ ahụ nke e wepụrụ na anụ ahụ site na slicer anụ oyi kpọnwụrụ akpọnwụ ga-etinye ya ozugbo n’ime ihe ndozi mmiri maka ndozi. Ọtụtụ mgbe, oke nke ụdị na fixative bụ 1: 4 ~ 20;
2. Steam fixation method: For relatively small and thick specimens, osmium acid or formaldehyde vapor fixation method can be used. For blood smears, osmium acid or formaldehyde vapor should be used to fix the blood before drying;
3. Mgbe ị na-eji slicer anụ oyi kpọnwụrụ maka slicing, ihe ndozi anyị na-ejikarị bụ 10% formaldehyde fixative na 95% ethanol fixative;
4. Injection and perfusion fixation method: some tissue blocks are too large or the fixative is difficult to penetrate into the interior, or the entire organ or the entire animal body needs to be fixed;
5. Adopt injection fixation or perfusion fixation method to inject the fixative into the blood vessel and reach the whole tissue and the whole body through the branch of the blood vessel, so as to obtain sufficient fixation.
Iji chịkọta ọnụ, nhọrọ na nhazi nke slicers anụ jụrụ oyi kwesịrị ịgbaso ụfọdụ ihe achọrọ. Akụkụ dị iche iche nwere ihe dị iche iche chọrọ. Ụzọ edozi dị iche iche chọrọ ihe dị iche iche na uru eke. N’otu aka ahụ, dị ka ụdị ihe na ọkpụrụkpụ nke anụ oyi kpọnwụrụ a ga-egbutu, a pụrụ iwere ihe ahụ ma dozie ya.