Relevant knowledge of material and fixation of mpitetika hena mangatsiaka

1. Fomba fanamboarana tavy kely: Fomba fampiasa matetika ity. Ny tavy kely nesorina tamin’ny biby tamin’ny alalan’ny slicer hena mangatsiaka dia tsy maintsy apetraka avy hatrany ao anaty fixative ranon-javatra ho an’ny fixation. Matetika, ny tahan’ny santionany amin’ny fixative dia 1: 4～20;

2. Steam fixation method: For relatively small and thick specimens, osmium acid or formaldehyde vapor fixation method can be used. For blood smears, osmium acid or formaldehyde vapor should be used to fix the blood before drying;

3. Rehefa mampiasa mpitety hena mangatsiatsiaka ho an’ny fitetika, dia ny fixative fampiasa matetika dia 10% formaldehyde fixative ary 95% ethanol fixative;

4. Injection and perfusion fixation method: some tissue blocks are too large or the fixative is difficult to penetrate into the interior, or the entire organ or the entire animal body needs to be fixed;

5. Adopt injection fixation or perfusion fixation method to inject the fixative into the blood vessel and reach the whole tissue and the whole body through the branch of the blood vessel, so as to obtain sufficient fixation.

Raha fintinina, ny fifantenana sy ny fametrahana ny slicers hena mangatsiaka dia mila manaraka ny fepetra sasany. Ny ampahany samihafa dia manana fepetra samihafa. Ny fomba fanamboarana samihafa dia mitaky fitaovana samihafa sy tombony voajanahary. Toy izany koa, araka ny karazana akora sy ny hatevin’ny hena mangatsiaka hotapahana, dia azo alaina sy raikitra ny akora.