Relevant knowledge of material and fixation of umkhenkce wokusika inyama

1. Indlela yokulungisa izicubu ezincinci: Le yindlela esetyenziswa ngokuqhelekileyo. Ithishu encinci ekhutshwe kwisilwanyana ngumceli wenyama engumkhenkce kufuneka ifakwe ngokukhawuleza kwindawo yolwelo ukuze ilungiswe. Ngokuqhelekileyo, umlinganiselo wesampulu kwi-fixative yi-1: 4～20;

2. Steam fixation method: For relatively small and thick specimens, osmium acid or formaldehyde vapor fixation method can be used. For blood smears, osmium acid or formaldehyde vapor should be used to fix the blood before drying;

3. Xa usebenzisa i-slicing yenyama ekhenkcezisiweyo ukusika, izilungiso zethu ezisetyenziswa ngokuqhelekileyo yi-10% ye-formaldehyde fixative kunye ne-95% ye-ethanol fixative;

4. Injection and perfusion fixation method: some tissue blocks are too large or the fixative is difficult to penetrate into the interior, or the entire organ or the entire animal body needs to be fixed;

5. Adopt injection fixation or perfusion fixation method to inject the fixative into the blood vessel and reach the whole tissue and the whole body through the branch of the blood vessel, so as to obtain sufficient fixation.

Ukushwankathela, ukukhethwa kunye nokulungiswa kweziceli zenyama efriziweyo kufuneka zilandele iimfuno ezithile. Amalungu ahlukeneyo aneemfuno ezahlukeneyo. Iindlela zokulungisa ezahlukeneyo zifuna izinto ezahlukeneyo kunye neenzuzo zendalo. Ngokufanayo, ngokohlobo lwezinto eziphathekayo kunye nobukhulu benyama enqabileyo ukuba inqunywe, izinto eziphathekayo zinokuthathwa kwaye zilungiswe.