Relevant knowledge of material and fixation of tranch vyann nan frizè

1. Tisi fiksasyon metòd: Sa a se yon metòd souvan itilize. Ti tisi a retire nan bèt la pa tranch vyann nan frizè a dwe imedyatman mete nan yon fiksatif likid pou fikse. Anjeneral, rapò echantiyon ak fiksatif se 1: 4 ~ 20;

2. Steam fixation method: For relatively small and thick specimens, osmium acid or formaldehyde vapor fixation method can be used. For blood smears, osmium acid or formaldehyde vapor should be used to fix the blood before drying;

3. Lè w ap itilize yon tranch vyann nan frizè pou tranche, fiksatif nou yo souvan itilize yo se 10% fiksasyon fòmaldeyid ak 95% fiksasyon etanòl;

4. Injection and perfusion fixation method: some tissue blocks are too large or the fixative is difficult to penetrate into the interior, or the entire organ or the entire animal body needs to be fixed;

5. Adopt injection fixation or perfusion fixation method to inject the fixative into the blood vessel and reach the whole tissue and the whole body through the branch of the blood vessel, so as to obtain sufficient fixation.

Pou rezime, seleksyon an ak fiksasyon nan tranch vyann nan frizè bezwen swiv sèten kondisyon. Pati diferan gen kondisyon diferan. Diferan metòd fikse mande pou diferan materyèl ak avantaj natirèl. Menm jan an tou, dapre kalite materyèl la ak epesè vyann nan frizè yo dwe koupe, materyèl la ka pran ak fiks.