Relevant knowledge of material and fixation of slicer daging beku

1. Cara fiksasi jaringan cilik: Iki minangka cara sing umum digunakake. Tisu cilik sing dicopot saka kewan kanthi ngiris daging beku kudu langsung diselehake ing fiksatif cair kanggo fiksasi. Biasane, rasio spesimen kanggo fiksatif yaiku 1: 4～20;

2. Steam fixation method: For relatively small and thick specimens, osmium acid or formaldehyde vapor fixation method can be used. For blood smears, osmium acid or formaldehyde vapor should be used to fix the blood before drying;

3. Nalika nggunakake slicer daging beku kanggo ngiris, fiksatif sing umum digunakake yaiku fiksatif formaldehida 10% lan fiksatif etanol 95%;

4. Injection and perfusion fixation method: some tissue blocks are too large or the fixative is difficult to penetrate into the interior, or the entire organ or the entire animal body needs to be fixed;

5. Adopt injection fixation or perfusion fixation method to inject the fixative into the blood vessel and reach the whole tissue and the whole body through the branch of the blood vessel, so as to obtain sufficient fixation.

Ing ringkesan, pilihan lan fiksasi slicers daging beku kudu ngetutake syarat tartamtu. Bagean sing beda duwe syarat sing beda. Cara ndandani sing beda-beda mbutuhake bahan sing beda lan kaluwihan alami. Kajaba iku, miturut jinis materi lan kekandelan daging beku sing bakal dipotong, materi kasebut bisa dijupuk lan didandani.