- 02
- Mar
Ujuzi unaofaa wa nyenzo na urekebishaji wa kipande cha nyama waliohifadhiwa
Relevant knowledge of material and fixation of kipande cha nyama waliohifadhiwa
1. Mbinu ya kurekebisha tishu ndogo: Hii ni njia inayotumiwa sana. Kitambaa kidogo kilichotolewa kutoka kwa mnyama na kipande cha nyama iliyohifadhiwa lazima kiweke mara moja kwenye fixative ya kioevu kwa ajili ya kurekebisha. Kawaida, uwiano wa specimen kwa fixative ni 1: 4~20;
2. Steam fixation method: For relatively small and thick specimens, osmium acid or formaldehyde vapor fixation method can be used. For blood smears, osmium acid or formaldehyde vapor should be used to fix the blood before drying;
3. Tunapotumia kipande cha kukata nyama iliyogandishwa kwa kukata, viambajengo vyetu vinavyotumika sana ni 10% ya kutengeneza formaldehyde na 95% ya kurekebisha ethanoli;
4. Injection and perfusion fixation method: some tissue blocks are too large or the fixative is difficult to penetrate into the interior, or the entire organ or the entire animal body needs to be fixed;
5. Adopt injection fixation or perfusion fixation method to inject the fixative into the blood vessel and reach the whole tissue and the whole body through the branch of the blood vessel, so as to obtain sufficient fixation.
Kwa muhtasari, uteuzi na urekebishaji wa vipande vya nyama waliohifadhiwa unahitaji kufuata mahitaji fulani. Sehemu tofauti zina mahitaji tofauti. Njia tofauti za kurekebisha zinahitaji vifaa tofauti na faida za asili. Vile vile, kwa mujibu wa aina ya nyenzo na unene wa nyama iliyohifadhiwa ya kukatwa, nyenzo zinaweza kuchukuliwa na kudumu.