- 02
- Mar
Muzlatilgan go’sht kesgichning materiali va fiksatsiyasi bo’yicha tegishli bilim
Relevant knowledge of material and fixation of muzlatilgan go’sht kesish mashinasi
1. Kichik to’qimalarni aniqlash usuli: Bu tez-tez ishlatiladigan usul. Muzlatilgan go’sht kesgich tomonidan hayvondan olib tashlangan mayda to’qimalarni mahkamlash uchun darhol suyuqlik fiksatoriga solib qo’yish kerak. Odatda, namunaning fiksatorga nisbati 1: 4~20;
2. Steam fixation method: For relatively small and thick specimens, osmium acid or formaldehyde vapor fixation method can be used. For blood smears, osmium acid or formaldehyde vapor should be used to fix the blood before drying;
3. Muzlatilgan go’shtni kesish uchun kesgichdan foydalanilganda, bizning tez-tez ishlatiladigan fiksatorlarimiz 10% formaldegid va 95% etanol fiksatoridir;
4. Injection and perfusion fixation method: some tissue blocks are too large or the fixative is difficult to penetrate into the interior, or the entire organ or the entire animal body needs to be fixed;
5. Adopt injection fixation or perfusion fixation method to inject the fixative into the blood vessel and reach the whole tissue and the whole body through the branch of the blood vessel, so as to obtain sufficient fixation.
Xulosa qilib aytadigan bo’lsak, muzlatilgan go’sht kesgichlarni tanlash va mahkamlash muayyan talablarga rioya qilish kerak. Turli qismlar turli talablarga ega. Turli xil mahkamlash usullari turli materiallar va tabiiy afzalliklarni talab qiladi. Xuddi shunday, materialning turiga va kesiladigan muzlatilgan go’shtning qalinligiga ko’ra, material olinishi va mahkamlanishi mumkin.