Relevant knowledge of material and fixation of isiceli senyama eqandisiwe

1. Indlela yokulungisa izicubu ezincane: Lena indlela esetshenziswa kakhulu. Izicubu ezincane ezikhishwa esilwaneni ngumsiki wenyama eqandisiwe kufanele zifakwe ngokushesha endaweni ewuketshezi ukuze zilungiswe. Ngokuvamile, isilinganiso se-specimen kuya kokulungisa singu-1: 4～20;

2. Steam fixation method: For relatively small and thick specimens, osmium acid or formaldehyde vapor fixation method can be used. For blood smears, osmium acid or formaldehyde vapor should be used to fix the blood before drying;

3. Uma sisebenzisa isisiki senyama eqandisiwe ukuze sisike, izithako zethu ezivame ukusetshenziswa zingu-10% we-formaldehyde fixative kanye no-95% we-ethanol fixative;

4. Injection and perfusion fixation method: some tissue blocks are too large or the fixative is difficult to penetrate into the interior, or the entire organ or the entire animal body needs to be fixed;

5. Adopt injection fixation or perfusion fixation method to inject the fixative into the blood vessel and reach the whole tissue and the whole body through the branch of the blood vessel, so as to obtain sufficient fixation.

Ukufingqa, ukukhethwa nokulungiswa kwezisiki zenyama eqandisiwe kudingeka kulandele izidingo ezithile. Izingxenye ezahlukene zinezidingo ezahlukene. Izindlela zokulungisa ezihlukene zidinga izinto ezahlukene kanye nezinzuzo zemvelo. Ngokufanayo, ngokusho kohlobo lwezinto ezibonakalayo kanye nobukhulu benyama efriziwe okufanele inqunywe, impahla ingathathwa futhi ilungiswe.