- 06
- Jan
Kev paub txog cov khoom siv thiab kho cov nqaij khov khov
Kev paub txog cov khoom siv thiab kho cov nqaij khov khov
Cov nqaij khov khov yog cov cuab yeej tshwj xeeb rau txiav cov nqaij khov. Nws yog siv hauv khw noj mov loj. Nws muaj kev ua haujlwm siab ntawm cov nqaij txiav thiab zoo abrasion tsis kam. Thaum ua haujlwm, feem ntau yuav tsum tau sau cov ntaub ntawv kom raug raws li cov khoom thiab cov qauv. Raws li cov khoom sib txawv thiab cov khoom, cov khoom siv tau tsim raws li qhov yuav tsum tau ua. Cov kev paub txog cov ntaub ntawv thiab fixation yog raws li nram no:
1. Txoj kev kho cov ntaub so ntswg me me: Qhov no yog ib txoj kev siv ntau. Cov ntaub so ntswg me me tshem tawm ntawm tus tsiaj los ntawm khov nqaij slicer yuav tsum tau muab tso rau tam sim ntawd rau hauv cov kua fixative rau fixation. Feem ntau, qhov piv ntawm cov qauv mus rau fixative yog 1: 4 ~ 20;
2. Steam fixation method: Rau cov qauv me me thiab tuab, osmium acid lossis formaldehyde vapor fixation txoj kev siv tau. Rau cov ntshav smears, osmium acid los yog formaldehyde vapor yuav tsum tau siv rau kho ua ntej cov ntshav smears qhuav;
3. Thaum siv cov nqaij khov khov rau slicing, peb cov khoom siv feem ntau yog 10% formaldehyde fixative thiab 95% ethanol fixative;
4. Kev txhaj tshuaj, perfusion fixation txoj kev: qee cov ntaub so ntswg loj dhau los yog cov kua dej fixative nyuaj rau nkag mus rau sab hauv, lossis tag nrho cov khoom nruab nrog cev lossis tag nrho cov tsiaj lub cev yuav tsum tau kho;
5. Siv kev txhaj tshuaj fixation los yog perfusion fixation, lub fixative yog txhaj rau hauv cov hlab ntsha, thiab cov hlab ntsha ceg mus rau tag nrho cov ntaub so ntswg thiab tag nrho lub cev, yog li tau txais fixation txaus.
Txhawm rau ua kom tiav, kev xaiv thiab kho cov nqaij khov khov yuav tsum ua raws li qee yam kev cai. Cov khoom sib txawv muaj qhov sib txawv. Cov kev kho sib txawv xav tau cov khoom sib txawv thiab cov txiaj ntsig zoo. Ib yam li ntawd, raws li cov khoom siv thiab cov tuab ntawm cov nqaij khov yuav raug txiav, cov khoom siv tau tuaj yeem raug coj mus thiab kho.