Ulwazi olufanelekileyo lwezinto kunye nokulungiswa kwesiceli senyama ekhenkcezisiweyo

Isixhobo sokusika inyama emkhenkceni sisixhobo esikhethekileyo sokusika inyama ekhenkcezileyo. Isetyenziswa kwiindawo zokutyela ezinkulu. Inomsebenzi ophezulu wokusika inyama kunye nokuchasana kakuhle kwe-abrasion. Xa usebenza, ngokuqhelekileyo kuyimfuneko ukuqokelela ngokuchanekileyo izinto eziphathekayo ngokweengxenye kunye neesampuli. Ngokwamacandelo ahlukeneyo kunye neempawu, izixhobo zisemgangathweni ngokweemfuno. Ulwazi olufanelekileyo malunga nezixhobo kunye nokulungiswa ngolu hlobo lulandelayo:

1. Indlela yokulungisa izicubu ezincinci: Le yindlela esetyenziswa ngokuqhelekileyo. Ithishu encinci ekhutshwe kwisilwanyana ngumceli wenyama engumkhenkce kufuneka ifakwe ngokukhawuleza kwindawo yolwelo ukuze ilungiswe. Ngokuqhelekileyo, umlinganiselo wesampulu kwi-fixative yi-1: 4～20;

2. Indlela yokulungisa umphunga: Kwimizekelo emincinci kunye nengqindilili, i-osmium acid okanye indlela yokulungisa umphunga i-formaldehyde ingasetyenziswa. Kwiimvavanyo zegazi, i-osmium acid okanye umphunga we-formaldehyde kufuneka isetyenziswe ukulungiswa phambi kokuba ii-smears zegazi zomiswe;

3. Xa usebenzisa i-slicing yenyama ekhenkcezisiweyo ukusika, izilungiso zethu ezisetyenziswa ngokuqhelekileyo yi-10% ye-formaldehyde fixative kunye ne-95% ye-ethanol fixative;

4. Isitofu, indlela yokulungiswa kwe-perfusion: ezinye iibhloko zezicubu zinkulu kakhulu okanye ulwelo olulungisiweyo lunzima ukungena ngaphakathi ngaphakathi, okanye i-organ yonke okanye umzimba wezilwanyana wonke kufuneka ulungiswe;

5. Ukusebenzisa ukulungiswa kwe-injection okanye ukulungiswa kwe-perfusion, i-fixative ifakwe kwi-blood vessel, kunye nesebe legazi legazi kwi-tissue yonke kunye nomzimba wonke, ngaloo ndlela ufumana ukulungiswa okwaneleyo.

Ukushwankathela, ukukhethwa kunye nokulungiswa kweziceli zenyama efriziweyo kufuneka zilandele iimfuno ezithile. Amalungu ahlukeneyo aneemfuno ezahlukeneyo. Iindlela zokulungisa ezahlukeneyo zifuna izinto ezahlukeneyo kunye neenzuzo zendalo. Ngokufanayo, ngokohlobo lwezinto eziphathekayo kunye nobukhulu benyama enqabileyo ukuba inqunywe, izinto eziphathekayo zinokuthathwa kwaye zilungiswe.